The Use of Attenuated Total Reflectance Infrared Spectroscopy for the Rapid Differentiation of Endospore-forming Bacteria

Download or read book The Use of Attenuated Total Reflectance Infrared Spectroscopy for the Rapid Differentiation of Endospore-forming Bacteria written by Elizabeth Marie Grasso and published by . This book was released on 2007 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: Endospore-forming bacteria, resistant to most sterilization and sanitation treatments, present a major challenge to the food industry through spoilage and food- borne disease. As cultural methods for detecting endospore-forming bacteria are intensive, rapid techniques are needed for identification, classification, and differentiation. Fourier transform infrared (FT-IR) spectroscopy provides physiochemical absorption spectra from fundamental vibrations, providing a reproducible and unique spectral fingerprint. Samples of Bacillus and Alicyclobacillus were measured using attenuated total reflectance (ATR) TR spectroscopy and microspectroscopy, to differentiate vegetative cells of these endospore-forming bacteria with minimal sample preparation. Mathematical transformations of the raw data increased separation through removal of baseline shifts, resolution of overlapping bands, and increased resolving power. Results indicated that soft independent modeling of class analogy (SIMCA) models, exhibited clusters that permitted accurate strain-level classification of all studied Bacillus and Alicyclobacillus isolates. The use of ATR-IR spectroscopy with multivariate analysis allows for an infrared screening procedure to complement elaborate molecular identification methods. This could become a powerful tool for the food industry and regulatory agencies for rapidly monitoring members of the Bacillus and Alicyclobacillus genus with regards to their impact in agriculture and public health.