Methods for Affinity-Based Separations of Enzymes and Proteins
Author | : Munishwar N. Gupta |
Publisher | : Birkhäuser |
Total Pages | : 234 |
Release | : 2013-12-01 |
ISBN-10 | : 9783034881272 |
ISBN-13 | : 3034881274 |
Rating | : 4/5 (72 Downloads) |
Download or read book Methods for Affinity-Based Separations of Enzymes and Proteins written by Munishwar N. Gupta and published by Birkhäuser. This book was released on 2013-12-01 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt: One major concern of biotechnology is either using enzymes or producing them. Enzyme/protein production is therefore an important starting point for biotechnology. Bioseparation or Downstream Processing constitutes about 40-90% of the total production cost. Driven by economics, highly selective technologies applicable to large-scale processing have emerged during the last decade. These technologies are slowly diffusing to enzymologists who are working on a smaller scale, looking for fast and efficient purification protocols. The affinity-based techniques (including precipitation, two-phase extractions, expanded bed chromatography, perfusion chromatography and monoliths) described in this volume provide current and new cutting-edge methods. Consequently, the book is of main interest to researchers in biochemistry, biochemical engineering and biotechnology, working either in academic or industrial sectors.