Development of a Multi-channel Visible-light Optical Coherence Tomography Platform for Investigating Fibrotic Disease Using Optical Scattering

Download or read book Development of a Multi-channel Visible-light Optical Coherence Tomography Platform for Investigating Fibrotic Disease Using Optical Scattering written by Ryan Niemeier and published by . This book was released on 2020 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Optical imaging and microscopy provide multi-dimensional information in both the laboratory and clinical settings. Novel imaging methods have leveraged several contrast sources in order to provide both qualitative and quantitative metrics. Optical coherence tomography (OCT) is a volumetric, micro-scale imaging modality which draws intrinsic contrast from the optical scattering of tissue. Visible-light OCT (VIS-OCT) increases the scattering contrast and is ideal for imaging thin layers of tissue with subtle changes to the optical scattering. The work contained in this dissertation is a modification of VIS-OCT to utilize simultaneous acquisition of multiple channels. Multi-channel VIS-OCT is capable of noise reduction, polarization sensitivity, and quantifying tissue optical scattering. The multi-channel system was used in several imaging applications with relevance to translational medical care and bench-top research. The system was used to image the optical scattering changes in esophageal tissue before and after microwave ablation. Measurements of optical scattering helped to identify the boundaries of the ablation which can provide valuable information both during treatment and for microwave and radiofrequency ablation antenna design. By adding polarization channels, multi-channel VIS-OCT was capable of measuring birefringence in a mouse model of pulmonary fibrosis which is being used to test novel therapeutics for lung disease. Finally, a variation of this OCT system was developed to be used in a multi-modal system which combines and co-registers several other imaging modalities and is well-suited to study fibrosis in glaucoma and cancer. The sensitivity of this VIS-OCT system to optical scattering and polarization effects make it a valuable tool with which to study fibrosis. This dissertation describes the framework of this platform and several utilities which in future work will incorporate a lens-free probe for cost-effective in vivo imaging and other bench-top variations will enable higher resolution imaging capable of capturing dynamic processes in live cells.